原標題：小鼠模型鼻內(nèi)給予益生菌鼠李糖乳桿菌GG可預防白樺花粉誘導的小過敏性哮喘
　　背景：益生菌在預防和治療氣道疾?。òㄟ^敏）方面的靶向應用越來越受到關注。在此，我們研究了益生菌鼠李糖乳桿菌gg和鼠李糖乳桿菌gr-1預防性鼻內(nèi)治療過敏性哮喘小鼠模型的有益效果。
　　方法：在第1-4天和第8-11天，以5x108 cfu/劑量鼻內(nèi)給藥鼠李糖乳桿菌8次，然后在兩周的哮喘誘導方案中交替給藥白樺花粉提取物。根據(jù)血清抗體水平、支氣管肺泡灌洗液細胞計數(shù)、肺組織學、肺細胞因子水平和氣道高反應性分析預防性治療的效果。通過鼻粘膜、糞便樣本、頸部淋巴結和血液中細菌細胞計數(shù)來評估鼠李糖乳桿菌的定植和易位。熒光顯微鏡觀察鼠李糖乳桿菌與固定的小鼠鼻粘膜細胞和氣道巨噬細胞的結合。
　　結果：鼠李糖乳桿菌（L.rhamnosusGG）短暫定植小鼠上呼吸道，其定植率約為鼠李糖乳桿菌（L.rhamnosusGR-1）的10倍?？梢姛晒馐罄钐侨闂U菌（L.rhamnosusGG）與小鼠鼻粘膜細胞和氣道巨噬細胞的明顯結合。用鼠李糖乳桿菌GG（而不是鼠李糖乳桿菌GR-1）進行預防性治療后，支氣管肺泡灌洗嗜酸細胞計數(shù)、肺白細胞介素-13和白細胞介素-5水平以及氣道高反應性顯著降低。同樣觀察到血清bet v 1-特異性Igg1下降的趨勢。
　　結論：鼻內(nèi)給予鼠李糖乳桿菌GG可以以一種特定的方式阻止樺樹花粉引起的過敏性哮喘的主要特征的發(fā)展。


延伸閱讀
Allergy                                                     
Intranasal administration of probiotic Lactobacillus rhamnosus GG prevents birch pollen-induced allergic asthma in a murine model 
DOI: 10.1111/all.13502
Abstract:
Background: There is an increasing interest in targeted application of probiotic bacteria for prevention and treatment of airway diseases, including allergies. Here, we investigated the beneficial effects of preventive intranasal treatment with probiotics Lactobacillus rhamnosus GG and L. rhamnosus GR-1 in a mouse model of allergic asthma.
Methods: L. rhamnosus was administered intranasally eight times on days 1-4 and 8-11 at 5x108 CFU/dose, followed by a two-week asthma induction protocol with birch pollen extract on alternating days. Effects of preventive treatment were analyzed based on serum antibody levels, bronchoalveolar lavage cell counts, lung histology, lung cytokine levels and airway hyperreactivity. Colonization and translocation of L. rhamnosus was assessed by bacterial cell counts in nasal mucosa, fecal samples, cervical lymph nodes and blood. Binding of fluorescent L. rhamnosus to fixed murine nasal mucosal cells and airway macrophages was visualized by fluorescence microscopy.
Results: Transient colonization of the murine upper airways by L. rhamnosus GG was demonstrated and was approximately ten times higher compared to L. rhamnosus GR-1.Marked binding of fluorescent L. rhamnosus GG to murine nasal mucosal cells and airway macrophages was visualized. Preventive treatment with L. rhamnosus GG (but not L.rhamnosus GR-1) resulted in a significant decrease in bronchoalveolar lavage eosinophil counts, lung interleukin-13 and interleukin-5 levels, and airway hyperreactivity. A tendency towards a decrease in serum Bet v 1-specific IgG1was likewise observed.
Conclusion: Intranasally administered L. rhamnosus GG prevents the development of cardinal features of birch pollen-induced allergic asthma in a strain-specific manner.
First Author:
Irina Spacova
Correspondence:
University of Antwerp, Department of Bioscience Engineering,Groenenborgerlaan 171, G.V.521, B-2020 Antwerp, Belgium
All Authors:
Irina Spacova, Mariya I. Petrova, Astrid Fremau, Lore Pollaris, JeroenVanoirbeek, Jan L. Ceuppens, Sven Seys, Sarah Lebeer
　　創(chuàng)建過敏性疾病的科研、科普知識交流平臺，為過敏患者提供專業(yè)診斷、治療、預防的共享平臺。