原標(biāo)題：國(guó)際鼻竇微生物組學(xué)研究：鼻腔細(xì)菌生態(tài)學(xué)的多中心、多民族特征
　　鼻竇微生物組的定義仍然不明確，我們目前的知識(shí)基于一些隊(duì)列研究，但這些研究結(jié)果并不一致。此外，鼻竇微生物組跨地域差異的變異性仍待探索。我們利用16S rRNA基因測(cè)序?qū)?lái)自世界各地的410人進(jìn)行了分析，確定了鼻竇微生物組及其在健康和疾病方面的地理變異。盡管個(gè)體之間的鼻竇微生物生態(tài)學(xué)差異很大，但我們確定了一個(gè)在健康和慢性鼻-鼻竇炎（CRS）人群中由棒狀桿菌、葡萄球菌、鏈球菌、嗜血桿菌和莫拉氏菌組成的核心微生物組。棒狀桿菌（平均相對(duì)豐度= 44.02%）和葡萄球菌（平均相對(duì)豐度= 27.34%）在大多數(shù)采樣患者中表現(xiàn)出明顯的優(yōu)勢(shì)。在患有鼻息肉的CRS患者中，發(fā)現(xiàn)棒狀桿菌的相對(duì)豐度在統(tǒng)計(jì)學(xué)上有顯著降低（40.29%比50.43%；P = .02）。盡管我們隊(duì)列中某些參與中心之間的微生物組組成和多樣性存在一定程度的測(cè)量差異，但這些差異不會(huì)改變所描述的核心生物體的總體模式。盡管如此，在短讀擴(kuò)增子測(cè)序研究中報(bào)告的非典型或異常生物，但不是核心微生物組的一部分，應(yīng)謹(jǐn)慎解釋。在我們的研究中描述的鼻竇微生物組的劃定和標(biāo)準(zhǔn)化方法將使鼻竇微生物群的進(jìn)一步表征和轉(zhuǎn)化應(yīng)用成為可能。


延伸閱讀
JACI
The international sinonasal microbiome study: A multicentre, multinational characterization of sinonasal bacterial ecology
DOI: 10.1111/all.14276
Abstract:
The sinonasal microbiome remains poorly defined, with our current knowledge based on a few cohort studies whose findings are inconsistent. Furthermore, the variability of the sinus microbiome across geographical divides remains unexplored. We characterize the sinonasal microbiome and its geographical variations in both health and disease using 16S rRNA gene sequencing of 410 individuals from across the world. Although the sinus microbial ecology is highly variable between individuals, we identify a core microbiome comprised of Corynebacterium, Staphylococcus, Streptococcus, Haemophilus and Moraxella species in both healthy and chronic rhinosinusitis (CRS) cohorts. Corynebacterium (mean relative abundance = 44.02%) and Staphylococcus (mean relative abundance = 27.34%) appear particularly dominant in the majority of patients sampled. Amongst patients suffering from CRS with nasal polyps, a statistically significant reduction in relative abundance of Corynebacterium (40.29% vs 50.43%; P = .02) was identified. Despite some measured differences in microbiome composition and diversity between some of the participating centres in our cohort, these differences would not alter the general pattern of core organisms described. Nevertheless, atypical or unusual organisms reported in short‐read amplicon sequencing studies and that are not part of the core microbiome should be interpreted with caution. The delineation of the sinonasal microbiome and standardized methodology described within our study will enable further characterization and translational application of the sinus microbiota.
First Author:
Sathish Paramasivan
Correspondence:
Alkis J. Psaltis, Department of Otolaryngology, Head and Neck Surgery, The Queen Elizabeth Hospital, 28 Woodville Road, Woodville South, SA 5011, Australia.
All Authors:
Sathish Paramasivan, Ahmed Bassiouni, Arron Shiffer, Matthew R. Dillon, Emily K. Cope, Clare Cooksley, Mahnaz Ramezanpour, Sophia Moraitis, Mohammad Javed Ali, Benjamin Bleier, Claudio Callejas, Marjolein E. Cornet, Richard G. Douglas, Daniel Dutra, Christos Georgalas, Richard J. Harvey, Peter H. Hwang, Amber U. Luong, Rodney J. Schlosser, Pongsakorn Tantilipikorn, Marc A. Tewfik, Sarah Vreugde, Peter-John Wormald, J. Gregory Caporaso, Alkis J. Psaltis
2020-09-03 Article 
　　創(chuàng)建過(guò)敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過(guò)敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。