原標(biāo)題：擴(kuò)大鮭魚和鲇魚的過(guò)敏原種類
背景：魚類過(guò)敏原的診斷測(cè)試受限于大量未研究的魚類。四種鮭魚過(guò)敏原是特征明確的，并在WHO/IUIS 進(jìn)行了注冊(cè)，而迄今為止尚未描述過(guò)鲇魚過(guò)敏原。2008年，淡水養(yǎng)殖鲇魚的產(chǎn)量超過(guò)了全球養(yǎng)殖最多的海洋魚類鮭魚。本研究旨在識(shí)別、量化和比較鮭魚和鲇魚中的所有 IgE 結(jié)合蛋白。
方法：77名經(jīng)臨床證實(shí)對(duì)魚類過(guò)敏的兒童患者接受了鮭魚和鲇魚的皮膚點(diǎn)刺試驗(yàn)。通過(guò)免疫印跡法對(duì)五種過(guò)敏原特異性抗體和患者的血清進(jìn)行實(shí)驗(yàn)，然后質(zhì)譜分析評(píng)估生的和加熱的蛋白質(zhì)提取物的過(guò)敏原庫(kù)。
結(jié)果：與鮭魚相比，鲇魚的生肉提取物和熟肉提取物顯示出更高的IgE結(jié)合頻率（分別為77%對(duì)70% 和64%對(duì)53%）。主要魚類過(guò)敏原小清蛋白顯示出最高的IgE結(jié)合能力 (10%-49%)，其次是生肉中的磷酸丙糖異構(gòu)酶 (TPI；19%-34%) 和熟肉提取物中的原肌球蛋白 (6%-32%)。包括TPI在內(nèi)的六種以前未識(shí)別的魚類過(guò)敏原已在 WHO/IUIS 注冊(cè)。分別有14%和10%的患者通過(guò)IgE檢測(cè)到來(lái)自鮭魚和鲇魚的肌酸激酶。鲇魚L-乳酸脫氫酶、3-磷酸甘油醛脫氫酶、丙酮酸激酶和葡萄糖-6-磷酸異構(gòu)酶顯示出會(huì)與6%-13%的患者出現(xiàn)IgE 結(jié)合。在鮭魚中，這些蛋白質(zhì)無(wú)法成功分離。
結(jié)論：本研究詳細(xì)介紹了兩種高度養(yǎng)殖的魚類的過(guò)敏原庫(kù)。
IgE與魚原肌球蛋白和TPI的結(jié)合首次在大型患者隊(duì)列中得到證實(shí)。
除了小清蛋白外，原肌球蛋白也應(yīng)考慮用于迫切需要改進(jìn)的魚類過(guò)敏原診斷測(cè)試中。


延伸閱讀
Allergy                                                  
Expanding the allergen repertoire of salmon and catfish
DOI: 10.1111/ALL.14574
Abstract:
Background: Diagnostic tests for fish allergy are hampered by the large number of under-investigated fish species. Four salmon allergens are well-characterized and registered with the WHO/IUIS while no catfish allergens have been described so far. In 2008, freshwater-cultured catfish production surpassed that of salmon, the globally most-cultured marine species. We aimed to identify, quantify, and compare all IgE-binding proteins in salmon and catfish.
Methods: Seventy-seven pediatric patients with clinically confirmed fish allergy underwent skin prick tests to salmon and catfish. The allergen repertoire of raw and heated protein extracts was evaluated by immunoblotting using five allergen-specific antibodies and patients' serum followed by mass spectrometric analyses.
Results: Raw and heated extracts from catfish displayed a higher frequency of IgE-binding compared to those from salmon (77% vs 70% and 64% vs 53%, respectively). The major fish allergen parvalbumin demonstrated the highest IgE-binding capacity (10%-49%), followed by triosephosphate isomerase (TPI; 19%-34%) in raw and tropomyosin (6%-32%) in heated extracts. Six previously unidentified fish allergens, including TPI, were registered with the WHO/IUIS. Creatine kinase from salmon and catfish was detected by IgE from 14% and 10% of patients, respectively. Catfish L-lactate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, and glucose-6-phosphate isomerase showed IgE-binding for 6%-13% of patients. In salmon, these proteins could not be separated successfully.
Conclusion: We detail the allergen repertoire of two highly farmed fish species. IgE-binding to fish tropomyosins and TPIs was demonstrated for the first time in a large patient cohort. Tropomyosins, in addition to parvalbumins, should be considered for urgently needed improved fish allergy diagnostics.
First Author:
Thimo Ruethers
Correspondence:
Andreas L. Lopata; Pharmacy and Medical Research, Bldg. 47,1 James Cook Drive, James Cook University, Townsville, QLD 4811, Australia
All Authors:
Thimo Ruethers, Aya C. Taki, Shaymaviswanathan Karnaneedi, Shuai Nie, Tanja Kalic, Danyi Dai, Sakda Daduang, Michael Leeming, Nicholas A. Williamson, Heimo Breiteneder, Sam S. Mehr, Sandip D. Kamath, Dianne E. Campbell, Andreas L. Lopata
2021-06-11 Article 
　　創(chuàng)建過(guò)敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過(guò)敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。