原標(biāo)題：盡管與Ara h6相似，Ara h2是仍是花生的主要過敏原
　?、俦尘埃篈ra h 2特異性IgE (sIgE)是目前診斷花生過敏的最佳血清學(xué)指標(biāo)。Ara h 6與Ara h 2共享60%的序列特性和多個(gè)表位。②目的：評價(jià)Ara h 2和Ara h 6對花生過敏的診斷價(jià)值和相對重要性。③方法：研究了花生過敏(PA)、花生致敏但耐受(PS)和非致敏非過敏(NA)兒童隊(duì)列(n=100)?；ㄉ蛡€(gè)別過敏原的sIgE水平采用 ImmunoCAP定量。分別對Ara h 2和Ara h 6進(jìn)行ImmunoCAP 抑制實(shí)驗(yàn)和肥大細(xì)胞活化試驗(yàn)(MAT)。采用SPSS v14和Prism v7進(jìn)行統(tǒng)計(jì)分析。④結(jié)果：Ara h 2 -sIgE和Ara h 6 -sIgE對花生過敏的診斷準(zhǔn)確性高于花生提取物和其他花生過敏原組分sIgE的診斷準(zhǔn)確性。大部分PA患者對Ara h 2和Ara h 6均敏感。Ara h2對Ara h 2 - sIgE結(jié)合的抑制作用大于Ara h 6 (p<0.001)，而Ara h 2和Ara h 6對Ara h 6 - sIgE結(jié)合的抑制作用相似(p=0.432)。在MAT上，當(dāng)檢測共敏個(gè)體時(shí)，Ara h 2誘導(dǎo)的最大反應(yīng)活性(p=0.001)明顯高于Ara h 6 而EC50 (p=0.002)明顯低于Ara h 6 。⑤結(jié)論：Ara h 2 - sIgE和Ara h 6 - sIgE診斷花生過敏的準(zhǔn)確性最高。在英國花生過敏中，盡管Ara h 2與Ara h 6存在一定程度的交叉反應(yīng)，但Ara h 2仍是主要的花生過敏蛋白。


延伸閱讀
JACI                                                  
Ara h 2 is the dominant peanut allergen despite similarities with Ara h 6
https://doi.org/10.1016/j.jaci.2020.03.026
Abstract
Background
Ara h 2 specific-IgE (sIgE) is to date the best serologic marker to diagnose peanut allergy. Ara h 6 shares ～60% sequence identity and multiple epitopes with Ara h 2.
Objectiv
To assess the diagnostic utility and relative importance of Ara h 2 and Ara h 6 in peanut allergy.
Methods
A cohort (n=100) of peanut allergic (PA), peanut sensitized but tolerant (PS) and non-sensitized non-allergic (NA) children were studied. sIgE levels to peanut and individual allergens were quantified using ImmunoCAP. ImmunoCAP inhibition experiments and mast-cell activation tests (MAT) were performed to both Ara h 2 and Ara h 6. Statistical analyses were performed using SPSS v14 and Prism v7.
Results
Ara h 2-sIgE and Ara h 6-sIgE showed the greatest diagnostic accuracy for peanut allergy compared with sIgE to peanut and other peanut allergens. Most PA patients were sensitized to both Ara h 2 and Ara h 6. Ara h 2 reduced Ara h 2-sIgE binding more than Ara h 6 (p<0.001) whereas Ara h 6-sIgE binding was inhibited to a similar degree by Ara h 2 and Ara h 6 (p=0.432). On the MAT, Ara h 2 induced significantly greater maximal reactivity (p=0.001) and lower EC50 (p=0.002) than Ara h 6 when testing co-sensitized individuals.
Conclusions
Ara h 2-sIgE and Ara h 6-sIgE provide the greatest accuracy for diagnosis peanut allergy. Ara h 2 is the dominant conglutin in peanut allergy in the UK, despite a degree of cross-reactivity with Ara h 6.
All authors:
OliverHemmings GeorgeDu ToitFRCPCH SuzanaRadulovic GideonLack FRCPCH Alexandra F.Santos
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。