原標(biāo)題：ImmunoCAP 分析：在變態(tài)反應(yīng)學(xué)中利弊
　　過敏原特異性IgE檢測及臨床病史是過敏診斷的基礎(chǔ)。在過去的幾十年里，過敏原提取物的特征描述和標(biāo)準(zhǔn)化及以檢測技術(shù)都得到了提高。在這里，我們討論了ImmunoCAP IgE檢測方法(Thermo Fisher Scientific/Phadia, Uppsala, Sweden)在變態(tài)反應(yīng)學(xué)領(lǐng)域的用途、優(yōu)點(diǎn)、誤解和局限性。它們可以進(jìn)行單一(ImmunoCAP)檢測，近十年來，可進(jìn)行多重(免疫固相變應(yīng)原芯片[ISAC])檢測。ImmunoCAP的主要優(yōu)點(diǎn)是獲得定量的過敏原特異性IgE抗體水平，且不受過敏原特異性IgG抗體的干擾。然而，ImmunoCAP過敏原提取物受提取物成分的限制。過敏原分子的引入對分析特異性和過敏診斷產(chǎn)生了重要影響。它們被用于單一ImmunoCAP和多重 ImmunoCAP ISAC檢測。ISAC的主要優(yōu)點(diǎn)是用微量血清獲得的綜合IgE圖譜。缺點(diǎn)是它只能半定量檢測，線性范圍較窄，以及每次檢測的成本較高。就檢測性能而言，ImmunoCAP過敏原提取物是很好的篩選工具，但過敏原分子在分子水平上分析IgE反應(yīng)，將過敏研究推向精準(zhǔn)醫(yī)學(xué)的位軌道。
延伸閱讀
JACI
ImmunoCAP assays: Pros and cons in allergology
https://doi.org/10.1016/j.jaci.2017.05.008 
Abstract:
Allergen-specific IgE measurements and the clinical history are the cornerstones of allergy diagnosis. During the past decades, both characterization and standardization of allergen extracts and assay technology have improved. Here we discuss the uses, advantages, misinterpretations, and limitations of ImmunoCAP IgE assays (Thermo Fisher Scientific/Phadia, Uppsala, Sweden) in the field of allergology.
They can be performed as singleplex (ImmunoCAP) and, for the last decade, as multiplex (Immuno Solid-phase Allergen Chip [ISAC]). The major benefit of ImmunoCAP is the obtained quantified allergen-specific IgE antibody level and the lack of interference from allergen-specific IgG antibodies. However, ImmunoCAP allergen extracts are limited to the composition of the extract. The introduction of allergen molecules has had a major effect on analytic specificity and allergy diagnosis.
They are used in both singleplex ImmunoCAP and multiplex ImmunoCAP ISAC assays. The major advantage of ISAC is the comprehensive IgE pattern obtained with a minute amount of serum. The shortcomings are its semiquantitative measurements, lower linear range, and cost per assay. With respect to assay performance, ImmunoCAP allergen extracts are good  screening tools, but allergen molecules dissect the IgE response on a molecular level and put allergy research on the map of precision medicine.
All Authors:
Mariannevan HageMD, PhDaCarlHamstenPhDaRudolfValentaMDb
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。