原標(biāo)題：室內(nèi)微生物群落:對(duì)兒童哮喘嚴(yán)重程度的影響
　?、賰和^敏性和非過敏性哮喘的嚴(yán)重程度可能受到微生物暴露的影響②我們?cè)噲D研究家庭微生物暴露與兒童哮喘嚴(yán)重程度之間的關(guān)系。③參與者(n = 196)是從康涅狄格州和馬薩諸塞州的一組哮喘兒童中挑選出來的。兒童按哮喘嚴(yán)重程度(輕度無癥狀或有最小癥狀需藥物治療或中度至重度持續(xù)性)和特應(yīng)性狀態(tài)(由血清IgE水平?jīng)Q定)進(jìn)行分組。采用下一代DNA測(cè)序和定量PCR法測(cè)定室內(nèi)粉塵中的微生物群落結(jié)構(gòu)和濃度。利用Logistic回歸研究哮喘嚴(yán)重程度與暴露指標(biāo)之間的關(guān)系，包括豐度、微生物種類鑒定和量化、群落組成、總真菌和細(xì)菌濃度。④在所有兒童中，除了使用距離比較t檢驗(yàn)的微生物群落組成外，還使用logistic回歸分析，結(jié)果顯示哮喘嚴(yán)重程度的增加與總變應(yīng)原性真菌種類濃度的增加、真菌總濃度的增加和細(xì)菌豐度的增加顯著相關(guān)。特應(yīng)性兒童哮喘的嚴(yán)重程度與真菌群落組成有關(guān)(P = .001)。通過logistic回歸分析，非變應(yīng)性患兒哮喘的嚴(yán)重程度與總真菌濃度相關(guān)(優(yōu)勢(shì)比，2.40;95% CI, 1.06-5.44)。真菌屬Volutella與特應(yīng)性兒童的哮喘加重有關(guān)(P = 0.0001, q = 0.04)。酵母屬Kondoa可能具有保護(hù)作用;隱球酵母也可能影響哮喘的嚴(yán)重程度。⑤在這一組兒童中，哮喘的嚴(yán)重程度與微生物暴露程度有關(guān)，并且根據(jù)周圍環(huán)境的不同而有不同的關(guān)聯(lián)。


延伸閱讀
JACI
[IF:13.1]
Indoor microbial communities: Influence on asthma severity in atopic and nonatopic children
https://doi.org/10.1016/j.jaci.2015.11.027
Abstract:
Background
Allergic and nonallergic asthma severity in children can be affected by microbial exposures.
Objective
We sought to examine associations between exposures to household microbes and childhood asthma severity stratified by atopic status.
Methods
Participants (n = 196) were selected from a cohort of asthmatic children in Connecticut and Massachusetts. Children were grouped according to asthma severity (mild with no or minimal symptoms and medication or moderate to severe persistent) and atopic status (determined by serum IgE levels). Microbial community structure and concentrations in house dust were determined by using next-generation DNA sequencing and quantitative PCR. Logistic regression was used to explore associations between asthma severity and exposure metrics, including richness, taxa identification and quantification, community composition, and concentration of total fungi and bacteria.
Results
Among all children, increased asthma severity was significantly associated with an increased concentration of summed allergenic fungal species, high total fungal concentrations, and high bacterial richness by using logistic regression in addition to microbial community composition by using the distance comparison t test. Asthma severity in atopic children was associated with fungal community composition (P = .001). By using logistic regression, asthma severity in nonatopic children was associated with total fungal concentration (odds ratio, 2.40; 95% CI, 1.06-5.44). The fungal genus Volutella was associated with increased asthma severity in atopic children (P = .0001, q = 0.04). The yeast genera Kondoa might be protective; Cryptococcus species might also affect asthma severity.
Conclusion
Asthma severity among this cohort of children was associated with microbial exposure, and associations differed based on atopic status.
All Author:
Karen C. Dannemiller  Janneane F. Gent Brian P. Leaderer Jordan Peccia
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。