原標(biāo)題：用300 IR舌下塵螨片免疫治療后的分子反應(yīng)譜顯示主要過敏原的顯著體液變化
背景：目前尚未有對舌下塵螨（HDM）片劑免疫療法治療患者的分子抗體反應(yīng)譜進行深入研究。本研究旨在使用300IR HDM舌下片進行過敏原特異性免疫治療（AIT）的雙盲安慰劑對照試驗中，通過過敏原微陣列技術(shù)對臨床定義的高應(yīng)答和低應(yīng)答患者進行體液免疫應(yīng)答研究。
方法：在100名表現(xiàn)出高或低臨床收益的受試者中，于基線和1年AIT后使用過敏原微陣列測量了血清中13種塵螨組分IgE、IgG和IgG4抗體水平。
結(jié)果：Der p 1、Der p 2和Der p 23是研究人群中最常見的過敏原組分。HDM相關(guān)哮喘患者Der p 1和Der p 23特異性IgE水平明顯高于其他組分。高應(yīng)答者和低應(yīng)答者之間的過敏原組分致敏分布沒有顯著差異。AIT時血清過敏原特異性IgG和IgG4增加，尤其是過敏原Der p1、Der p2和Der p 23（p<0.0001）。
結(jié)論：我們的研究人群確認Der p1和Der p23特異性IgE水平與哮喘相關(guān)。IgE反應(yīng)譜并不預(yù)示著舌下AIT的結(jié)果，300IR舌下片對單獨和多重致敏的受試者均有效。我們的研究首次證明了舌下AIT對HDM過敏原Der p1、Der p2和Der p2 3特異性IgG和IgG4的誘導(dǎo)作用。


原始出處
Allergy                                                  
Molecular reactivity profiling upon immunotherapy with a 300 IR sublingual house dust mite tablet reveals marked humoral changes towards major allergens
DOI: 10.1111/all.15327
Abstract:
Background: Molecular antibody reactivity profiles have not yet been studied in depth in patients treated by sublingual house dust mite (HDM) tablet immunotherapy. Humoral immune responses to a large panel of HDM mite allergens were studied using allergen microarray technology in a subset of clinically defined high and low responder patients from a double-blind placebo-controlled allergen-specific immunotherapy (AIT) trial using sublingual 300 IR HDM tablets.
Methods: : Serum levels of IgE, IgG and IgG4 to 13 Dermatophagoides pteronyssinus molecules were measured at baseline and after 1-year AIT, using allergen microarrays in 100 subjects exhibiting high or low clinical benefit.
Results: Der p 1, Der p 2 and Der p 23 were the most frequently recognized allergens in the study population. Patients with HDM-related asthma had significantly higher allergen-specific IgE levels to Der p 1 and Der p 23. No significant difference in the distribution of allergen sensitization pattern was observed between high and low responders. An increase in serum allergen-specific IgG and IgG4 occurred upon AIT, in particular to allergens Der p 1, Der p 2 and Der p 23 (p < 0.0001).
Conclusion: We confirm for our study population that Der p 1-and Der p 23-specific IgE levels are associated with asthma. IgE reactivity profiles were not predicitive of sublingual AIT outcomes, with 300 IR tablets as efficacious in pauci-and multi-sensitized subjects. Our study is the first to demonstrate the induction of IgG and IgG4 specific for the HDM allergens Der p 1, Der p 2 and Der p 23 by sublingual AIT.
First Author:
Ekaterina Potapova
Correspondence:
Laurent Mascarell, Innovation & Science Department, Stallergenes SAS, Antony, France.
2023-02-01 Article