原標(biāo)題：螨蟲(chóng)主要過(guò)敏原Der p 23的血清學(xué)、基因組學(xué)和結(jié)構(gòu)分析
　?、僮罱跉W洲人群中發(fā)現(xiàn)Der p23是主要過(guò)敏原和潛在的甲殼素結(jié)合蛋白。②本研究旨在評(píng)估Der p23在北美人群中的重要性，以及確定功能性特征的結(jié)構(gòu)。③用ELISA法檢測(cè)IgE與Der p23、Der p1、Der p2、Der p5、Der p7、Der p8的結(jié)合情況。螨蟲(chóng)RNA-seq數(shù)據(jù)被比作是過(guò)敏原表達(dá)水平的估計(jì)。用x射線晶體學(xué)和核磁共振對(duì)其結(jié)構(gòu)進(jìn)行了分析。盡管Der p23的致敏率較高(達(dá)到75%、Der p 1 和 Der p 2分別為87%和79%)，但抗Der p23 IgE水平相對(duì)較低。④患者對(duì)6種過(guò)敏原的反應(yīng)是可變的(n = 47)，但平均抗der p1和抗der p2占特異性IgE的85%。在豐度方面，Der p23的RNA表達(dá)水平是主要過(guò)敏原中最低的，比Der p1低30倍，比Der p2低7倍。Der p23的結(jié)構(gòu)是一個(gè)小的球狀蛋白，由兩個(gè)二硫化物鍵穩(wěn)定，結(jié)構(gòu)上與Blo t12等過(guò)敏原相關(guān)，這些過(guò)敏原含有與甲殼素結(jié)合的碳水化合物結(jié)合域。功能性實(shí)驗(yàn)未能證實(shí)甲殼素與Der p23結(jié)合。⑤IgE對(duì)螨蟲(chóng)變應(yīng)原的應(yīng)答主要由Der p1和Der p2引起的，Der p23只占其中很小的比例。與其他塵螨過(guò)敏原的表達(dá)相比，Der p23和Der p2的致敏率和特異性IgE的數(shù)量是異常高的。


延伸閱讀
Clinical & Experimental Allergy
Serological, genomic and structural analyses of the major mite allergen Der p 23.
doi: 10.1111/cea.12680
Background
Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein.
Objective
This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization.
Methods
IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR.
Results
Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n = 47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23.
Conclusions and Clinical Relevance
Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.
All Authors:
G A. Mueller1 , T. A. Randall2 , J. Glesner3 , L. C. Pedersen1 , L. Perera1 , L. L. Edwards1 , E. F. DeRose1 , M. D. Chapman3 , R. E. London1 and A. Pomes3 1 Genome Integrity and Structural Biology Laboratory, Research Triangle Park, NC, USA, 2 Integrative Bioinformatics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA and 3 INDOOR Biotechnologies, Inc., Charlottesville, VA, USA
　　創(chuàng)建過(guò)敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過(guò)敏患者提供專(zhuān)業(yè)診斷、治療、預(yù)防的共享平臺(tái)。