Background: Cat allergy is a major trigger of asthma worldwide. Molecular patterns of cat sensitisationvary between individuals, but their relationship to inflammation in asthmatics has not beenextensively studied. Methods: Patients with asthma (age 10?35 years; n=266) and IgE sensitisation to cat, dog or horse extract (ImmunoCAP), were analysed for IgE to the cat allergen components Fel d 1 (secretoglobin), Fel d 2 (serum albumin), Fel d 4 and Fel d 7 (lipocalins). Independent associations between IgE-antibody concentrations, and fraction of exhaled nitric oxide (FeNO), blood eosinophil (B-Eos) count, and total IgE were analysed by multiple linear regression after adjustment for possible confounders.

Background: Screening for specific IgE against 2S albumin proteins Ara h 2 and 6 has good positive predictive value in diagnosing peanut allergy. From the third 2S member Ara h 7, 3 isoforms have been identified. Their allergenicity has not been elucidated.

Background:Shellfish allergy is one of the commonest food allergies in the tropics. Methods:Serum allergen-specific IgE of 105 subjects were quantified using ImmunoCAP and ImmunoCAP ISAC biochips. The subjects were classified based on a convincing clinical history and food challenge testing (FC) (dose=70g) to Penaeus monodon and Litopenaeus vannamei. Group1A: Either SA with FC positive to either shrimp (n =22) or SA admitted to emergency departments for severe reactions but no FC performed (n=14) (total n=36); and Group1B: Reported SA with FC negative (n=31). Group2: Shellfish tolerant DM sensitized controls (n= 38).