杭州浙大迪迅生物基因工程有限公司Release date：2019-06-06
JACI
[IF:13.1]
High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens
DOI: https://doi.org/10.1016/j.jaci.2017.12.910
Abstract:
Rationale
House dust mites (HDM) are a predominant source of inhalant allergens that attribute to over 50% of worldwide allergy cases, while the full spectrum of HDM allergens remains unknown. Here we sequenced a high-quality genome of Dermatophagoides (D.) pteronyssinus to find known canonical allergens and allergen orthologs inferred from D. farinae genome.
Methods
The D. pteronyssinus genome was assembled by a hybrid assembly approach using PacBio, Illumina and Ion Torrent reads. Transcriptomes of D. pteronyssinus and D. farinae were compared using Cufflinks. Allergens were identified by two-dimensional gel electrophoresis and MALDI-ToF MS. Allergenicities of the novel allergens were detected by ELISA using patients’ sera against bacteria expressing recombinant proteins.
Results
Genomic, transcriptomic, and proteomic approaches revealed full gene structures of 21 known allergens, and uncovered 11 putative allergen homologs. A high-quality D. pteronyssinus genome of 66.8 Mb was constructed. This genome assembly represented 98.2% of estimated genome size, with contig N50 being 80kb. The first comprehensive transcriptomic analysis of D. pteronyssinus and D. farinae revealed distinctively expressed allergen genes between the two dust mites, in which Der p 1, 5, 10, 21 and 24 were highly expressed in D. pteronyssinus, while Der f 10, 13, 21, 26 and 31 expressed higher in D. farinae. From ELISA, IgE binding rates of Der p 25, 26, 28, 32, and 33 were 38.5-65.3%, in which Der p 33 had the highest allergenicity.
Conclusions
Bioinformatics and experimental characterizations of D. pteronyssinus genome and allergens provide important resources for future investigation of HDM allergens
All Author:
Angel Tsz-Yau Wan Xiao-Yu Liu Kevin Yi Yang Mingqiang Wang Jamie Sui-Lam Kwok Zhiyuan Yang Xiao-Jun Xiao Carol Po-Ying Lau Ying Li Zhi-ming Huang Jin-ge Ba Aldrin Kay-Yuen Yim Chun-Yan Ouyang Sai-Ming Ngai x
Sai-Ming Ngai
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Ting-Fung Chan Elaine Lai-Han Leung x
Elaine Lai-Han Leung
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Liang Liu Zhi-Gang Liu Stephen Kwok-Wing Tsui
[IF:13.1]
High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens
DOI: https://doi.org/10.1016/j.jaci.2017.12.910
Abstract:
Rationale
House dust mites (HDM) are a predominant source of inhalant allergens that attribute to over 50% of worldwide allergy cases, while the full spectrum of HDM allergens remains unknown. Here we sequenced a high-quality genome of Dermatophagoides (D.) pteronyssinus to find known canonical allergens and allergen orthologs inferred from D. farinae genome.
Methods
The D. pteronyssinus genome was assembled by a hybrid assembly approach using PacBio, Illumina and Ion Torrent reads. Transcriptomes of D. pteronyssinus and D. farinae were compared using Cufflinks. Allergens were identified by two-dimensional gel electrophoresis and MALDI-ToF MS. Allergenicities of the novel allergens were detected by ELISA using patients’ sera against bacteria expressing recombinant proteins.
Results
Genomic, transcriptomic, and proteomic approaches revealed full gene structures of 21 known allergens, and uncovered 11 putative allergen homologs. A high-quality D. pteronyssinus genome of 66.8 Mb was constructed. This genome assembly represented 98.2% of estimated genome size, with contig N50 being 80kb. The first comprehensive transcriptomic analysis of D. pteronyssinus and D. farinae revealed distinctively expressed allergen genes between the two dust mites, in which Der p 1, 5, 10, 21 and 24 were highly expressed in D. pteronyssinus, while Der f 10, 13, 21, 26 and 31 expressed higher in D. farinae. From ELISA, IgE binding rates of Der p 25, 26, 28, 32, and 33 were 38.5-65.3%, in which Der p 33 had the highest allergenicity.
Conclusions
Bioinformatics and experimental characterizations of D. pteronyssinus genome and allergens provide important resources for future investigation of HDM allergens
All Author:
Angel Tsz-Yau Wan Xiao-Yu Liu Kevin Yi Yang Mingqiang Wang Jamie Sui-Lam Kwok Zhiyuan Yang Xiao-Jun Xiao Carol Po-Ying Lau Ying Li Zhi-ming Huang Jin-ge Ba Aldrin Kay-Yuen Yim Chun-Yan Ouyang Sai-Ming Ngai x
Sai-Ming Ngai
Search for articles by this author
Ting-Fung Chan Elaine Lai-Han Leung x
Elaine Lai-Han Leung
Search for articles by this author
Liang Liu Zhi-Gang Liu Stephen Kwok-Wing Tsui
2019-4-30 Artical
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